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Image Search Results
Journal: Nutrients
Article Title: Fatty Acid-Derived N -acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model
doi: 10.3390/nu14183879
Figure Lengend Snippet: Effects of LPS and DS treatment on microglial morphology, proinflammatory cytokines, and NO production. ( a ) Representative images of SIM-A9 microglia cell culture after LPS (1 μg/mL) and DS (0.1, 1 and 10 μg/mL) treatment, scale bar—100 μm. ( b ) Western blot analysis of SIM-A9 microglia cell lysates ( a ) INFγ (25 kDa), TNFα (26 kDa), IL-1β (30 kDa), IL6 (22 kDa), and MHCII (47 kDa) were detected in LPS-activated SIM-A9 cell lysates with β-tubulin protein expression used as a loading control. Original uncropped images of blots are shown in . ( c ) Relative quantity of INFγ, TNFα, IL-1β, IL6, and MHCII in SIM-A9 microglia cell lysates determined with Western blot, n = 4, * p < 0.001—compared to Veh, + p < 0.001—compared to LPS. ( d ) Nitrite production was detected with the Griess test, n = 6 per group. Data are presented as mean ± SEM, +++ p < 0.001 — compared to LPS, *** p < 0.001 — compared to Veh.
Article Snippet: For in vitro studies, we used a
Techniques: Cell Culture, Western Blot, Expressing